General Cell Lines and Cell Cultures
ATCC Methylomicrobium album genomic DNA
Genomic DNA isolated from Methylomicrobium album strain NCIB 11123. This bacterial strain is also available as ATCC Catalog No.: 33003™.
Eurofins Discoverx Ready-to-Assay α2B Adrenergic Receptor Frozen Cells, 2 Vials of Cells. (HTS157LRTA)
The Ready-to-Assay α2B Adrenergic Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. epinephrine and norepinephrine) based activation of ADRA2B GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses ADRA2B alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of ADRA2B, the resulting calcium activity can be assayed in either detection mode. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen MC1 Melanocortin Receptor Membrane Preparation, 1 vial. (HTS156M)
The ChemiScreen MC1 Melanocortin Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of MC1R surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx Ready-to-Assay M3 Acetylcholine (Muscarinic) Receptor Frozen Cells, 2 Vials of Cells. (HTS116RTA)
The Ready-to-Assay M3 Acetylcholine (Muscarinic) Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. acetylcholine) based activation of CHRM3 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CHRM3 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of CHRM3, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx Ready-to-Assay S1P2 Lysophospholipid Receptor Frozen Cells, 2 Vials of Cells. (HTS078RTA)
The Ready-to-Assay S1P2 Lysophospholipid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. S1P) based activation of S1PR2 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses S1PR2 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of S1PR2, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx Ready-to-Assay M4 Acetylcholine (Muscarinic) Receptor Frozen Cells, 2 Vials of Cells. (HTS117RTA)
The Ready-to-Assay M4 Acetylcholine (Muscarinic) Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. acetylcholine) based activation of CHRM4 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CHRM4 alongside exogenous levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of CHRM4, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
ATCC Enterobacter hormaechei; 6 x 0.3mL
ATCC 700323-MINI-PACK™ consists of 6 ready-to-use vials of ATCC 700323™ frozen in 200 μL of glycerol stock, eliminating the need to rehydrate and culture the strain prior to use. Each vial is provided with a 2-D barcode for easy storage and tracking, as well as peel-off labels for fast and reliable recordkeeping.
ATCC Exophiala; dermatitidis; purified DNA 2ug
Genomic DNA isolated from Exophiala dermatitidis 8656 [CBS 525.76]. This fungal strain is also available as ATCC Catalog No.: 34100™.
ATCC Lactobacillus jensenii; genomic DNA
Genomic DNA isolated from Lactobacillus jensenii strain 62G. This bacterial strain is also available as ATCC Catalog No. 25258™.
Eurofins Discoverx ChemiScreen α1B Adrenergic Receptor Stable Cell Line, 2 vials. (HTS158C)
The ChemiScreen α1B Adrenergic Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. epinephrine and norepinephrine) based activation of ADRA1B GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses ADRA1B alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of ADRA1B, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen β3 Adrenoceptor Receptor Membrane Preparation, 1 vial. (HTS159M)
The ChemiScreen β3 Adrenoceptor Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of ADRB3 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx Ready-to-Assay Glucagon Receptor Frozen Cells, 2 Vials of Cells. (HTS112RTA)
The Ready-to-Assay Glucagon Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. glucagon) based activation of GCGR GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses GCGR alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of GCGR, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.